FIG. 1.

ARV-176 shows a predilection for macrophages. Diluted stocks of ARV-138 and ARV-176 were used to infect QM5 fibroblasts (a and b, respectively). Infected cell monolayers were incubated at 37°C and fixed at 16 hpi before being immunostained using a reovirus-specific rabbit polyclonal antiserum and goat anti-rabbit immunoglobulin G conjugated with alkaline phosphatase to detect viral foci of infection. Virus dilutions that gave equivalent numbers of foci in QM5 fibroblasts were then used to infect HD-11 macrophages (c and d) that were similarly fixed and immunostained at 9 hpi.