FIG. 5.

ARV-176 and ARV-138 protein synthesis and replication are similar in fibroblasts and macrophages. (A) Cell monolayers of quail QM5 fibroblasts or HD-11 macrophages were left uninfected (U) or infected with ARV-176 (176) or ARV-138 (138) using the minimum concentrations of virus inocula required for complete infection of the monolayer (see Fig. 4). Cultures were pulse-labeled for 1 h with [³⁵S]methionine at 12 and 16 hpi, and the radiolabeled cell lysates were fractioned by SDS-PAGE and detected by autoradiography. The locations of the major λ, μ, and ς classes of viral proteins are indicated on the right. (B) Monolayers of quail QM5 fibroblasts or HD-11 macrophage cells were infected with ARV-176 (gray bars) or ARV-138 (black bars) as described for panel A. Infected cultures were harvested at 48 hpi, and the yield of infectious progeny virions was determined by a plaque assay on QM5 cell monolayers. Results are presented as means and standard errors from a representative experiment.