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The ARV-176 M2 genome segment is required for enhanced macrophage infection. Virus stocks of ARV-176 (a and b), ARV-138 (c and d), and the ARV-138 M2 monoreassortant R402 (e and f) were standardized to give equivalent concentrations of focus-forming units in quail QM5 fibroblasts (a, c, and e). The same virus dilutions were used to infect HD-11 macrophage monolayers (b, d, and f). Cells were immunostained as described for Fig. 1 to reveal viral foci of infection.
