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. 2024 Oct 20;16:60. doi: 10.1038/s41368-024-00323-x

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© The Author(s) 2024, corrected publication 2024

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Fig. 5 — Inhibition of intracellular-, and not extracellular membrane bound, Nogo-A is responsible for gene expression modulation. a Immunofluorescent staining showing expression of Nogo-A (red colour) and Hsp90 (green colour) in LS8 cells. White colour: phalloidin; blue colour: DAPI. Immunofluorescent staining showing surface-bound (b) and total (c) Nogo-A (red colour) in LS8 cells. Blue colour: DAPI. d Quantification of correlated total cell fluorescence in (b) and (c). e Experimental strategy to determine whether cell membrane-bound or intracellular Nogo-A mediates the regulation of ameloblastic differentiation genes. f Real-time PCR analysis of the expression of Amelogenin (Amelx), Ameloblastin (Ambn), Enamelin (Enam) and Kallikrein 4 (Klk4) upon antibody-mediated inhibition of membrane-bound Nogo-A (α-Nogo-A IgG, magenta line) or overall Nogo-A knockdown (shRNA α-Nogo-A, light blue line). Gene expression is expressed as a fold-change over the 36B4 housekeeping gene. The graph represents the mean and standard deviation (n = 3)