Table 8.
Statistical Information of Selected gene and protein expression in HCM3 following PA + CORT treatment after FKBP5 SiRNA in Fig. 8. Sample size: n = 6 rats/group.
| TNF-α |
TNF-α |
NF-κB |
NF-κB |
|
|---|---|---|---|---|
| Gene expression |
Protein release |
Gene expression |
% Activation |
|
| Source of Variation | F Statistic, posthoc | F Statistic, post-hoc | F Statistic, post-hoc | F Statistic, post-hoc |
| SiRNA | F (1, 24) = 59.42, p < 0.0001 | F (1, 24) = 16.71, p = 0.0004 | F (1, 24) = 30.54, p < 0.0001 | F (1, 24) = 59.82, p < 0.0001 |
| Treatment | F (3, 24) = 19.85, p < 0.0001 | F (3, 24) = 3.926, p = 0.0206 | F (3, 24) = 38.97, p < 0.0001 | F (3, 24) = 9.359, p = 0.0003 |
| Treatment:SiRNA | F (3, 24) = 12.28, p < 0.0001 | F (3, 24) = 7.273, p = 0.0012 | F (3, 24) = 20.54, p < 0.0001 | F (3, 24) = 3.372, p = 0.0350 |
| VEH:Ctrl siRNA vs. VEH:FKBP5 siRNA | p = 0.0324 | p = 0.9997 | p = 0.9998 | p = 0.3867 |
| VEH:Ctrl siRNA vs. Cort:Ctrl siRNA | p = 0.7881 | p = 0.3417 | p = 0.6369 | p = 0.0535 |
| VEH:Ctrl siRNA vs. Cort:FKBP5 siRNA | p = 0.0015 | p > 0.9999 | p > 0.9999 | p = 0.9807 |
| VEH:Ctrl siRNA vs. PA:Ctrl siRNA | p = 0.0003 | p = 0.9760 | p = 0.0045 | p = 0.0198 |
| VEH:Ctrl siRNA vs. PA:FKBP5 siRNA | p = 0.0003 | p > 0.9999 | p > 0.9999 | p = 0.6359 |
| VEH:Ctrl siRNA vs. PA Cort:Ctrl siRNA | p = 0.0093 | p = 0.0004 | p < 0.0001 | p = 0.0012 |
| VEH:Ctrl siRNA vs. PA Cort:FKBP5 siRNA | p = 0.0031 | p = 0.9999 | p = 0.5043 | p = 0.9995 |
| VEH:FKBP5 siRNA vs. Cort:Ctrl siRNA | p = 0.5102 | p = 0.6236 | p = 0.8774 | p = 0.0003 |
| VEH:FKBP5 siRNA vs. Cort:FKBP5 siRNA | p = 0.8935 | p > 0.9999 | p = 0.9940 | p = 0.0749 |
| VEH:FKBP5 siRNA vs. PA:Ctrl siRNA | p = 0.5498 | p = 0.9997 | p = 0.0015 | p < 0.0001 |
| VEH:FKBP5 siRNA vs. PA:FKBP5 siRNA | p = 0.5037 | p > 0.9999 | p > 0.9999 | p = 0.9999 |
| VEH:FKBP5 siRNA vs. PA Cort:Ctrl siRNA | p < 0.0001 | p = 0.0012 | p < 0.0001 | p < 0.0001 |
| VEH:FKBP5 siRNA vs. PA Cort:FKBP5 siRNA | p = 0.9709 | p = 0.9838 | p = 0.2649 | p = 0.6974 |
| Cort:Ctrl siRNA vs. Cort:FKBP5 siRNA | p = 0.0538 | p = 0.4609 | p = 0.4506 | p = 0.3051 |
| Cort:Ctrl siRNA vs. PA:Ctrl siRNA | p = 0.0130 | p = 0.8808 | p < 0.0001 | p = 0.9998 |
| Cort:Ctrl siRNA vs. PA:FKBP5 siRNA | p = 0.0109 | p = 0.4224 | p = 0.7040 | p = 0.0008 |
| Cort:Ctrl siRNA vs. PA Cort:Ctrl siRNA | p = 0.0002 | p = 0.0818 | p < 0.0001 | p = 0.7430 |
| Cort:Ctrl siRNA vs. PA Cort:FKBP5 siRNA | p = 0.0997 | p = 0.1714 | p = 0.0176 | p = 0.0169 |
| Cort:FKBP5 siRNA vs. PA:Ctrl siRNA | p = 0.9980 | p = 0.9944 | p = 0.0094 | p = 0.1406 |
| Cort:FKBP5 siRNA vs. PA:FKBP5 siRNA | p = 0.9960 | p > 0.9999 | p = 0.9999 | p = 0.1687 |
| Cort:FKBP5 siRNA vs. PA Cort:Ctrl siRNA | p < 0.0001 | p = 0.0007 | p < 0.0001 | p = 0.0114 |
| Cort:FKBP5 siRNA vs. PA Cort:FKBP5 siRNA | p > 0.9999 | p = 0.9980 | p = 0.6918 | p = 0.8308 |
| PA:Ctrl siRNA vs. PA:FKBP5 siRNA | p > 0.9999 | p = 0.9908 | p = 0.0035 | p = 0.0003 |
| PA:Ctrl siRNA vs. PA Cort:Ctrl siRNA | p < 0.0001 | p = 0.0040 | p = 0.0001 | p = 0.9359 |
| PA:Ctrl siRNA vs. PA Cort:FKBP5 siRNA | p = 0.9800 | p = 0.8601 | p = 0.3071 | p = 0.0059 |
| PA:FKBP5 siRNA vs. PA Cort:Ctrl siRNA | p < 0.0001 | p = 0.0006 | p < 0.0001 | p < 0.0001 |
| PA:FKBP5 siRNA vs. PA Cort:FKBP5 siRNA | p = 0.9694 | p = 0.9999 | p = 0.4386 | p = 0.9017 |
| PA Cort:Control siRNA vs. PA Cort:FKBP5 siRNA | p < 0.0001 | p = 0.0001 | p < 0.0001 | p = 0.0003 |