Table 3.

Comparison of assumptions required by detectCilia and detection results.

Additional medium components	Mean nucleus area in µm2	Mean cilium projection area in µm2	Ciliated cells in %
ITS	$11.9\times 11.9$	1.9	67
ITS with Dexa	$12.8\times 12.8$	1.9	84
ITS with Dexa + IGF + TGF	$14.1\times 14.1$	1.5	65
FBS	$13.9\times 13.9$	1.5	72

The assumptions were nucleus area in the order of 15 μm × 15 μm, cilia projection area between 0.5 μm² and 5 μm², and (at most) 1 cilium per cell. Note that cilia touching a z border were included in this table.