FIG. 4.

Effects of TRAF expression on oriP activity. Shown are the results of a transient replication assay of the oriP plasmid. (A) Effects of single expression of TRAF. The oriP plasmid (2 μg), the control plasmid (0.5 μg), and the TRAF expression plasmid (4 μg) were transfected. Total amounts of plasmids were 6.5 μg. (B) Effects of coexpression of TRAFs. The oriP plasmid (2 μg), the control plasmid (0.5 μg), and the TRAF expression plasmids (4 μg each) were transfected. Total amounts of plasmids were 10.5 μg. (C) Effects of coexpression of TRAF and the dominant negative TRAF mutants, TRAF5DN and TRAF6DN. Normalized amounts of DpnI-resistant plasmid are shown below. Data represent averages of three experiments with standard errors (SE). Two fragments indicated by asterisks are products of replication intermediates.