FIG. 5.

Effects of dominant negative TRAF expression on oriP activity. Shown are the results of a transient replication assay of the oriP plasmid. (A) Effects of dominant negative TRAF (TRAFDN) in the absence of LMP1 expression. The oriP plasmid (2 μg), the control plasmid (0.5 μg), and the expression plasmids of TRAFDN (4 μg each) were transfected. Total amounts of plasmids were 10.5 μg. (B) Effects of dominant negative TRAFDN on LMP1-induced suppression. The oriP plasmid (2 μg), the control plasmid (0.5 μg), the TRAF expression plasmids (4 μg each), and LMP1Δ(212-386) (0.25 μg) were transfected. Total amounts of plasmids were 10.75 μg. Normalized amounts of DpnI-resistant plasmid are shown at the bottom. Data represent averages of three experiments with standard errors (SE). Two fragments indicated by asterisks are products of replication intermediates.