FIG. 7.

LPS simulation resulted in loss of Akata EBV. (A) LPS stimulation of EBV-infected B cell lines. Cells (10⁵ cells/ml) were stimulated with 10 μg of LPS/ml for 2 or 4 days. Total DNA of these stimulated and unstimulated cells (4 μg) was digested with BamHI and was analyzed by Southern blot hybridization using an EBV(B95-8) BamHI-C fragment for a probe, which cross-hybridized with BamHI-W (3.1 kb). For the loading controls, EtBr-staining images of agarose gels are shown in the middle panel. Hybridization signals were measured and shown as a relative copy number in the lower panel. Raji and Daudi EBVs were replicated by the DS-independent mechanism (16). (B) The effects of SB208350 on EBV replication. EBV-infected B cell lines (10⁵/ml) were cultured in the presence of the specific inhibitor for p38 MAPK SB208350 (20 μM) with or without LPS stimulation (5 μg/ml) for 4 days. Total DNA (4 μg) was prepared and analyzed as described above for panel A. Hybridization signals of the BamHI-C fragment are shown in the left panels. EtBr-staining images of agarose gels are shown in the right panels.