Fig. 6.

Measurement of MPO activity and NE levels in lung lysates prepared from the lungs of exposed-mice infected with NTHI for 0, 4, and 12 h. Switching from cigarettes to HTP did not modulate MPO activity whereas cessation was beneficial (A) and switching augmented lung NE levels (B). Difference between groups is considered significant at p < 0.05 using the Kruskal-Wallis test corrected for multiple comparisons by controlling the False Discovery Rate using Two-stage linear step-up procedure of Benjamini, Krieger and Yekuieli post-test. Desired FDR was adjusted at Q = 0.1. *Statistically significant difference from continued exposure to cigarettes (blue). # Statistically significant difference from cessation (green). Numbers of mice used for MPO assay: continued exposure to CS group (n = 8 mice at 0, n = 8 mice at 4 h and n = 7 mice at 12 h timepoint); for switching to HTP group (n = 8 mice at each 0, 4 and 12 h timepoints), and for cessation group (n = 8 mice at each 0, 4 and 12 h timepoints). Numbers of mice used for NE assay: continued exposure to CS group (n = 8 mice at 0, n = 10 mice at 4 h and n = 8 mice at 12 h timepoint); for switching to HTP group (n = 10 mice at each 0, 4 and 12 h timepoints), and for cessation group (n = 8 mice at 0, n = 10 mice at 4 h and n = 8 mice at 12 h timepoint). Results are depicted as mean ± SE