Fig 2.

Dronedarone hydrochloride bound directly to SRC kinase

(A) Computational docking model between DH and SRC. (B–E) DH directly binds to SRC. The recombinant proteins (B) or cell lysates of HEK293F (C), HGC27 (D), and AGS (E) cells were incubated with DH-conjugated Sepharose 4B beads or with Sepharose 4B beads alone. The results were analyzed by Western blot. (F, G) The binding capacity of DH to SRC in gastric cancer intact cells. The HGC27 (F) and AGS (G) cells were treated with DH or DMSO for 24 h and at different temperatures. The protein bindings were visualized by Western blot.