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. Author manuscript; available in PMC: 2024 Oct 21.
Published in final edited form as: Sci Immunol. 2024 Sep 20;9(99):eadl3755. doi: 10.1126/sciimmunol.adl3755

Figure 2. Optimized 2-shot prime immunization amplifies the GC response and trimer-specific serum antibody titers over time compared to bolus immunization.

Figure 2.

(A) Schematic of dosing schemes. (B-H) C57BL/6J mice (n=9 animals/group) were immunized with 10 μg N332-GT2 trimer and 5 μg SMNP adjuvant according to the dosing schemes in (A). GC responses were evaluated on days 7, 14, 21, and 28 by flow cytometry and antibody responses by ELISA on days 3, 7, 14, 21, and 28. Shown are trimer-specific B cell counts (B), GC B cell counts (C), Tfh cell counts (D), trimer-specific GC B cell counts (E), plasmablast counts (F), trimer-specific IgM titers (G), and trimer-specifc IgG titers (H), plotted over time for bolus and 2-ED regimens. Shown are data from one independent experiment for each immunization series. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, not significant; by two-way ANOVA with Dunnett’s multiple comparison post test compared to bolus immunization.