Figure 4.

Mutation of srrAB sensitizes Staphylococcus aureus USA400 MW2 to R91.A, MIC of R91 against two-component system mutants in an S. aureus USA400 MW2 background. Data are shown as the mean ± SD from at least three biological replicates. ∗∗∗∗p ≤ 0.0001 using a one-way ANOVA with Dunnett’s multiple comparisons. B, MIC of the S. aureus USA400MW2 background strain, ΔsrrAB, complement (psrrAB), and empty vector (pEmpty). Data are shown as the mean ± SD from at least three biological replicates. ∗p ≤ 0.05, ∗∗∗p ≤ 0.001 using a one-way ANOVA with a Šidák multiple comparison. C, MIC of ΔmenD and terminal electron acceptor mutants (qoxA::tn and ΔcydAB) in a ΔmenD background. MIC was determined in TSB after 24 h of growth using concentrations that completely inhibited growth, as determined by measurement of A₆₀₀. Data are shown as the mean ± SD from at least three biological replicates. ∗∗∗p ≤ 0.001 using a one-way ANOVA with a Šidák multiple comparison.