TABLE 1.

Primers used in vIL-8 analysis

Gene	Primer sequences	Product (bp)	Reference
Chicken β-actin	ACTF4: 5′-CCATGAAACTACCTTCAACTCCA-3′	628 (unspliced)
	ACTR4: 5′-GATTCATCGTACTCCTGCTTGCT-3′	274 (spliced)	30
gC	gCF: 5′-TACTCTGCGATACGGAAG-3′	584	31
	gCR: 5′-GAGGAGTTGCAATGTTTCC-3′
GFP	GFPF: 5′-CTTGTTGAATTAGATGGTGATGTT-3′	584	17
	GFPR: 5′-TTTCGAAAGGG-CAGATTGTG-3′
ICP27	ICP27F: 5′-GCAAGAAGGGCATCACCGAAGAA-3′	670	56
	ICP27R: 5′-ACGACGAATGCGCGATAACAAAAT-3′
Meq	MEQF: 5′-CCCCTTCCCTGACGGCCTATCTGA-3′	421	27
	MEQR: 5′-CTGGGCGCAAAGTTCCTCCGTATC-3′
RRL	RRLF: 5′-GCCGCCGTTGCTATTTCTCTA-3′	549	31
	RRLR: 5′-ATGACGCGTGATGCCAACAAC-3′
vIL-8 (His tagged)	5vIL-8NHE: 5′-GCACGCTAGCCGCCACCATGCAGGCGTTGTTGCTAG-3′	845	This report
	vIL-8HIS: 5′-CGGGATCCTAGTGATGATGATGGTGATGAAGACAGATATGGGA-3′
vIL-8 (structural analysis)	vIL-8 no. 1: 5′-GGAGACCCAATAAC-AGGGAAATCGC-3′	939	This report
	vIL-8 no. 4: 5′-CGACAGACAGTTGTACACCTGCCTG-3′
vIL-8 (RT-PCR)	vIL8F: 5′-TGCAGGCGTTGTTG-CTAGTATTGGTT-3′	699 (unspliced)	This report
	vIL8R: 5′-ACAGGAGGTAGCAATTAATCAAAGAGA-3′	423 (spliced)
vIL-8 (SS)	5′-CGTAGGTTACCGTATCGGATAG-3′	See Fig. 2	This report
vIL-8 (vIL-8B1)	5′-GGTCCACACATACCTTCCTGTTCTTC-3′	See Fig. 2	This report
vIL-8 (SS-dT)	5′-CGTAGGTTACCGTATCGGATAGCGGCCGCATTTTTTTTTTTTTTTTTT-3′	See Fig. 2	This report
vIL-8 (vIL-8F4)	5′-GCAAGTGCGTGAAAGTCACTAATCG-3′	See Fig. 2	This report