FIG. 7.

Triton X-114 phase-partitioning analysis of VP40 and its deletion mutant proteins. 293 cells were transfected with the appropriate plasmid DNA, and at 48 h posttransfection, the cells were collected in TN buffer and disrupted by Dounce homogenization. The homogenate was partitioned into aqueous (A) and detergent (D) phases as described in Materials and Methods. Proteins were separated by SDS–12% PAGE and analyzed by Western blotting.