Table 1.
Signal intensities (mean ± SD of triplicates) of product 3 after derivatization of substrates with different derivatization agents and co-solvents.
| Substrate (5µM) | Derivatization agent | Co-Solvent | Retention Time | Signal intensity (105) of respective product 3 |
|---|---|---|---|---|
| Guanidine | Benzoin | Methoxyethanol | 3.75 | 70.7 ± 4.5 |
| Methylguanidine | Benzoin | Methoxyethanol | 3.80 | 110 ± 13 |
| Arginine | Benzoin | Methoxyethanol | 3.50 | 7.0 ± 1.1 |
| Guanidine | Anisoin | Methoxyethanol | 3.82 | 28.8 ± 4.0 |
| Guanidine | Benzoin | Ethanol | 3.78 | 63.9 ± 1.4 |
| Methylguanidine | Benzoin | Ethanol | 3.83 | 72 ± 11 |
| Arginine | Benzoin | Ethanol | 3.53 | 5.58 ± 0.39 |