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. 1988 Jul 1;253(1):281–285. doi: 10.1042/bj2530281

A slow kinetic transient in RNA synthesis catalysed by wheat-germ RNA polymerase II.

C Job 1, L De Mercoyrol 1, D Job 1
PMCID: PMC1149287  PMID: 2458718

Abstract

Progress curves of U-A-primed RNA synthesis catalysed by wheat-germ RNA polymerase II on a poly[d(A-T)] template exhibit a slow burst of activity. In contrast, the progress curves of single-step addition of UMP to U-A primer in the abortive elongation reaction do not exhibit the slow burst of activity. The correlation between the kinetic transient in the productive pathway of RNA synthesis and the rate of abortive elongation is suggestive of the occurrence of a slow conformational change of the transcription complex during the transition from abortive to productive elongation. The exceptional duration of the transient burst (in the region of 4 min) may suggest a transition of a hysteretic type.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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