Table 1.
Screening of CNS depressant activity of MDML, its solvent fraction, and MDMS stem barks by calculating the mean number of movements of mice in hole cross test
| Group | Dose [mg/kg] | Number of movements of mice (% inhibition of movements) | ||||
|---|---|---|---|---|---|---|
| pretreatment | 30 min | 60 min | 90 min | 120 min | ||
| Control | – | 13.4 ± 1.5 | 12 ± 1.41 | 11.4 ± 1.36 | 11.2 ± 1.16 | 11.4 ± 1.03 |
| Diazepam | 1 | 14.2 ± 1.77 | 6.4 ± 1.25* (46.67%) | 3.6 ± 0.81*** (68.42%) | 1 ± 0.32*** (91.07%) | 0.6 ± 0.24*** (94.74%) |
| MDML | 200 | 15 ± 1.22 | 7 ± 1.3* (41.67%) | 5.4 ± 0.68*** (52.63%) | 2.2 ± 0.37*** (80.36%) | 1.4 ± 0.51*** (87.72%) |
| 400 | 11.2 ± 1.2 | 5.4 ± 0.6** (55%) | 4.2 ± 0.49*** (63.15%) | 2.2 ± 0.2*** (80.36%) | 1.2 ± 0.37*** (89.47%) | |
| MDHL | 200 | 12.6 ± 1.5 | 10.2 ± 1.43 (15%) | 6.2 ± 0.97** (45.61%) | 3.4 ± 0.87*** (69.64%) | 1.6 ± 0.51*** (85.96%) |
| 400 | 12 ± 0.95 | 7.6 ± 0.75 (36.67%) | 5.4 ± 0.81*** (52.63%) | 1.4 ± 0.6*** (87.5%) | 1 ± 0.45*** (91.23%) | |
| MDTL | 200 | 14.8 ± 0.66 | 9 ± 0.89 (25%) | 6.6 ± 0.24** (42.11%) | 3.8 ± 0.49*** (66.07%) | 3 ± 0.55*** (73.68%) |
| 400 | 15.2 ± 1.16 | 7.2 ± 1.07 (40%) | 4.8 ± 0.8*** (57.89%) | 1.8 ± 0.58*** (83.93%) | 1 ± 0.45*** (91.23%) | |
| MDCL | 200 | 13.6 ± 1.21 | 9.6 ± 1.36 (20%) | 4.8 ± 0.97*** (57.89%) | 3 ± 0.71*** (73.21%) | 1.6 ± 0.51*** (85.96%) |
| 400 | 15.6 ± 1.08 | 8 ± 1.41 (33.33%) | 4.2 ± 0.97*** (63.16%) | 1.2 ± 0.58*** (89.29%) | 0.6 ± 0.24*** (94.74%) | |
| MDEL | 200 | 13.4 ± 1.21 | 9 ± 0.95 (25%) | 6 ± 0.95*** (47.37%) | 2.6 ± 0.51*** (76.79%) | 1.8 ± 0.37*** (84.21%) |
| 400 | 16.2 ± 1.53 | 8.6 ± 1.81 (28.33%) | 3.6 ± 0.68*** (68.42%) | 1 ± 0.55*** (91.07%) | 0.6 ± 0.4*** (94.74%) | |
| MDAL | 200 | 16 ± 1.22 | 8.6 ± 1.03 (28.33%) | 4.6 ± 0.98*** (59.65%) | 2.2 ± 0.37*** (80.36%) | 1 ± 0.45*** (91.23%) |
| 400 | 16.2 ± 1.24 | 7.4 ± 1.12 (38.33%) | 3.2 ± 0.87*** (71.93%) | 1.8 ± 0.58*** (83.93%) | 0.8 ± 0.37*** (92.98%) | |
| MDMS | 200 | 14.6 ± 1.82 | 9.8 ± 0.66 (18.33%) | 6.6 ± 0.75** (42.11%) | 3.6 ± 0.51*** (67.86%) | 2 ± 0.45*** (82.46%) |
| 400 | 13.6 ± 1.57 | 6.66 ± 0.93* (45%) | 4.2 ± 0.73*** (63.16%) | 2.4 ± 0.4*** (78.57%) | 1.8 ± 0.58*** (84.21%) | |
MDML – crude methanol extract of M. dispermus leaves, MDHL – n -hexane fraction of crude methanol extract of M. dispermus leaves, MDTL – carbon tetrachloride fraction of crude methanol extract of M. dispermus leaves, MDCL – chloroform fraction of crude methanol extract of M. dispermus leaves, MDEL – ethyl acetate fraction of crude methanol extract of M. dispermus leaves, MDAL – aqueous fraction of crude methanol extract of M. dispermus leaves, MDMS – crude methanol extract of M. dispermus stem barks; results were expressed as mean ± SEM;
P < 0.05,
P < 0.01,
P < 0.001 were considered statistically significant compared to control