Table 2.
CNS depressant activity of the investigated extracts of M. dispermus leaves and stem barks by using the rotarod test
| Group | Dose [mg/kg] | Falling time of mice in seconds (% of reduction in falling time) | ||||
|---|---|---|---|---|---|---|
| pretreatment | 30 min | 60 min | 90 min | 120 min | ||
| Control | – | 180 ± 0 | 180 ± 0 (0%) | 179.316 ± 1.42 (0.38%) | 173.168 ± 1.14 (3.8%) | 170.72 ± 1.11 (5.16%) |
| Diazepam | 1 | 180 ± 0 | 33.188 ± 4.75*** (81.56%) | 24.938 ± 6.17*** (86.15%) | 13.928 ± 2.06*** (92.26%) | 9.322 ± 0.65*** (94.82%) |
| MDML | 200 | 180 ± 0 | 156.83 ± 6.84 (12.87%) | 82.052 ± 4.95*** (54.42%) | 68.08 ± 4.16*** (62.18%) | 30.538 ± 1.4*** (83.03%) |
| 400 | 180 ± 0 | 62.896 ± 5.08*** (65.06%) | 53.238 ± 7.37*** (70.42%) | 41.904 ± 4.92*** (76.72%) | 29.602 ± 2.66*** (83.55%) | |
| MDHL | 200 | 180 ± 0 | 171.4 ± 4.25 (4.78%) | 164.524 ± 6.73 (8.6%) | 148.726 ± 7.67** (17.37%) | 110. 226 ± 5.14*** (38.76%) |
| 400 | 180 ± 0 | 170.542 ± 3.85 (5.25%) | 147.83 ± 4.73** (17.87%) | 127.822 ± 2.64*** (28.99%) | 84.926 ± 3.18*** (52.82%) | |
| MDTL | 200 | 180 ± 0 | 58.83 ± 7.83*** (67.32%) | 38.664 ± 7.01*** (78.52%) | 31.92 ± 5.33*** (82.27%) | 18.152 ± 2.5*** (89.92%) |
| 400 | 180 ± 0 | 26.81 ± 6.52*** (85.11%) | 23.444 ± 5.67*** (86.98%) | 22.068 ± 5.34*** (87.74%) | 11.02 ± 0.85*** (93.88%) | |
| MDCL | 200 | 180 ± 0 | 138.234 ± 7.9*** (23.2%) | 124.478 ± 3.72*** (30.85%) | 84.364 ± 6.35*** (53.13%) | 61.662 ± 2.92*** (65.74%) |
| 400 | 180 ± 0 | 33.84 ± 3.49*** (81.2%) | 22.09 ± 4.19*** (87.72%) | 13.67 ± 0.96*** (92.41%) | 9.566 ± 0.45*** (94.69%) | |
| MDEL | 200 | 180 ± 0 | 157.214 ± 7.45 (12.66%) | 131.798 ± 6.9*** (26.78%) | 95.114 ± 7.78*** (47.16%) | 72.014 ± 4.46*** (59.99%) |
| 400 | 180 ± 0 | 65.706 ± 6.7*** (63.5%) | 44.5 ± 4.43*** (75.28%) | 36.974 ± 7.26*** (79.46%) | 17.12 ± 1.83*** (90.49%) | |
| MDAL | 200 | 180 ± 0 | 48.67 ± 5.92*** (72.96%) | 41.612 ± 4.74*** (76.88%) | 33.548 ± 1.17*** (81.36%) | 19.148 ± 1.8*** (89.36%) |
| 400 | 180 ± 0 | 27.442 ± 2.25*** (84.75%) | 20.558 ± 3.25*** (88.58%) | 13.344 ± 2.7*** (92.59%) | 9.698 ± 0.98*** (94.61%) | |
| MDMS | 200 | 180 ± 0 | 161.672 ± 5.72 (10.18%) | 147.752 ± 5.61*** (17.92%) | 117.032 ± 4.85*** (34.98%) | 111.606 ± 4.87*** (37.99%) |
| 400 | 180 ± 0 | 161.802 ± 6.24 (10.67%) | 122.662 ± 2.76*** (31.85%) | 109.154 ± 3.88*** (39.36%) | 92.748 ± 0.9*** (48.47%) | |
MDML – crude methanol extract of M. dispermus leaves, MDHL – n -hexane fraction of crude methanol extract of M. dispermus leaves, MDTL – carbon tetrachloride fraction of crude methanol extract of M. dispermus leaves, MDCL – chloroform fraction of crude methanol extract of M. dispermus leaves, MDEL – ethyl acetate fraction of crude methanol extract of M. dispermus leaves, MDAL – aqueous fraction of crude methanol extract of M. dispermus leaves, MDMS – crude methanol extract of M. dispermus stem barks; results were expressed as mean ± SEM; *P < 0.05,
P < 0.01,
P < 0.001 were considered statistically significant compared to control