Fig. 1. Amino acid substitution profiles associated with n = 181 blaSHV alleles. (a) Positions studied in the article by Neubauer et al. and tracked in Kleborate v2 are shown as columns; these include sites where substitutions have a clear association with ESBL activity [148, 179, 238 and 240, omega-loop (positions 164–178)] or BLI resistance activity (69, 234 and 235), plus some sites (25, 35, 146 and 156) that are associated with increased MIC to ceftaroline but not ceftriaxone or inhibitor resistance. Position 179 is also a part of the omega-loop and is specifically separated to show its association with ESBL profiles. Position 130 is not included, as it is found only in SHV-10 (BLI resistant) for which there is no nt sequence available. Each row indicates a unique combination of aas across these variable sites. The aas present in SHV-1 are indicated in grey at the top of the panel, where the omega-loop sequence (Ω) is RWETELNEALPGDARD. (b) The number of unique nt alleles associated with each aa profile (row) is shown as a barplot. Colours indicate the functional class assigned to these alleles, on the basis of the mutations shown here and supporting literature (cited in the text and in Table S1).
