Table 2.
Central composite design (CCD) and response values for hyoscyamine and scopolamine extraction.
| Run | A: Methanol (%) |
B: Temperature (°C) |
C: Ultrasonication time (min) |
Hyoscyamine (μg/g DW) |
Scopolamine (μg/g DW) |
||
|---|---|---|---|---|---|---|---|
| Actual Value | Predicted Value | Actual Value | Predicted Value | ||||
| 1 | 75 (+1) | 40 (+1) | 40 (+1) | 27.13 | 30.98 | 104.21 | 113.91 |
| 2 | 50 (0) | 45 (+2) | 30 (0) | 69.34 | 71.99 | 86.51 | 76.98 |
| 3 | 25 (−1) | 40 (+1) | 40 (+1) | 48.56 | 44.01 | 55.58 | 66.92 |
| 4 | 75 (+1) | 30 (−1) | 40 (+1) | 61.17 | 53.59 | 87.56 | 89.21 |
| 5 | 50 (0) | 25 (−2) | 30 (0) | 49.32 | 52.04 | 126.1 | 140.51 |
| 6 | 0 (−2) | 35 (0) | 30 (0) | 19.71 | 19.42 | 13.44 | 18.16 |
| 7 | 50 (0) | 35 (0) | 30 (0) | 41.58 | 33.83 | 102.77 | 95.54 |
| 8 | 50 (0) | 35 (0) | 30 (0) | 35.55 | 33.83 | 107.82 | 95.54 |
| 9 | 75 (+1) | 30 (−1) | 20 (−1) | 65.34 | 64.52 | 165.98 | 149.75 |
| 10 | 25 (−1) | 30 (−1) | 20 (−1) | 9.56 | 0.3442 | 111.41 | 96.83 |
| 11 | 50 (0) | 35 (0) | 50 (+2) | 32.65 | 30.7 | 108.84 | 97.32 |
| 12 | 100 (+2) | 35 (0) | 30 (0) | 64.89 | 70.56 | 117.89 | 118.06 |
| 13 | 50 (0) | 35 (0) | 10 (−2) | 33.20 | 40.52 | 83.14 | 99.54 |
| 14 | 50 (0) | 35 (0) | 30 (0) | 21.00 | 33.83 | 96.86 | 95.54 |
| 15 | 75 (+1) | 40 (+1) | 20 (−1) | 90.67 | 78.5 | 118.2 | 117.86 |
| 16 | 50 (0) | 35 (0) | 30 (0) | 32.52 | 33.83 | 100.65 | 95.54 |
| 17 | 25 (−1) | 30 (−1) | 40 (+1) | 31.25 | 38.05 | 103.11 | 98.56 |
| 18 | 50 (0) | 35 (0) | 30 (0) | 37.10 | 33.83 | 79.54 | 95.54 |
| 19 | 50 (0) | 35 (0) | 30 (0) | 29.87 | 33.83 | 80.74 | 95.54 |
| 20 | 25 (−1) | 40 (+1) | 20 (−1) | 40.71 | 42.91 | 15.14 | 8.61 |
The bold value indicates the maximum value.