Table 3.

ANOVA results for the response surface quadratic model for optimization of extraction parameters.

Source	df	Hyoscyamine			Scopolamine
		Mean Square	F-value	p-value	Mean Square	F-value	p-value
Model	9	745.24a	10.11	0.0006	2331.12a	10.63	0.0005
A-Methanol	1	2615.58a	35.47	0.0001	9980.11a	45.50	<0.0001
B-Temperature	1	397.87a	5.40	0.0426	4035.68a	18.40	0.0016
C-Ultrasonication time	1	96.44 ns	1.31	0.2794	4.93 ns	0.0225	0.8838
AB	1	408.37a	5.54	0.0404	1587.04a	7.24	0.0227
AC	1	1182.22a	16.03	0.0025	1938.84a	8.84	0.0140
BC	1	669.80a	9.08	0.0130	1601.04a	7.30	0.0222
A2	1	195.64 ns	2.65	0.1344	1183.05a	5.39	0.0426
B2	1	1248.45a	16.93	0.0021	273.99 ns	1.25	0.2898
C2	1	4.98 ns	0.0675	0.8003	13.09 ns	0.0597	0.8120
Residual	10	73.74			219.32
Lack of Fit	5	97.31 ns	1.94	0.2424	298.24 ns	2.12	0.2140
Pure Error	5	50.17			140.41
Cor Total	19
R2		0.9009			0.9054
CV (%)		20.42			15.88

^aindicates significant and ^ns indicates non-significant.