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. 2024 Oct 3;77:103387. doi: 10.1016/j.redox.2024.103387

Fig. 1.

Fig. 1

Exosomes from LPS-treated mast cells induced ferroptosis and inflammation in HBE cells. HBE cells were incubated with the conditioned medium of HMC-1 cells stimulated with LPS or PBS or exosomes, with or without additional LPS treatment. The experiments were conducted with three independent technical replicates. (A) Flowchart illustrating the experimental procedure. (B) Exosome characterization. Representative images for TEM (upper left), NTA (upper right), and western blots for the exosomal markers CD81 and ALIX (lower panel). (C) Representative images of the exosome uptake experiment. (D) qPCR results for IL6 and TNFa. (E) qPCR results for GPX4, ACSL4, and ALOX15. ∗∗∗P < 0.001 (ANOVA, followed by multiple comparison tests). ACSL4, long-chain acyl-CoA synthetase 4; ALOX15, 15-lipoxygenase; GPX4, glutathione peroxidase 4; HBE, human bronchial epithelial; LPS, lipopolysaccharide; NTA, nanoparticle tracking analysis; PBS, phosphate-buffered saline; TEM, transmission electron microscopy; LPS-MC-CM, conditioned medium from HMC-1 cells stimulated with LPS; PBS-MC-CM, conditioned medium from HMC-1 cells stimulated with PBS; LPS-MC-EX, exosome from HMC-1 cells stimulated with LPS; PBS-MC-EX, exosome from HMC-1 cells stimulated with PBS.