Fig. 7.

Anta-miR-744 transfection affected HBE cell viability, apoptosis, and migration via GPX4, ACSL4, and ALOX15 regulation. HBE cells were analyzed after transfection with anta-miR-744, with or without the corresponding inhibitors. The experiments were performed with three independent technical replicates. (A) CCK-8 assay results. (B) Flow cytometry results. Left panel: Representative flow cytometry images; Right panel: Quantification of flow cytometry images. (C) Representative images of Transwell migration assays. (D) Representative images of scratch assay. (E) qPCR results for IL6 and TNFa expression. ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001 (ANOVA, followed by multiple comparison tests). ACSL4, long-chain acyl-CoA synthetase 4; ALOX15, 15-lipoxygenase; GPX4, glutathione peroxidase 4; HBE, human bronchial epithelial; miRNA, microRNA.