Fig 1. Treatment with NHC inhibits LACV replications more potently than RBV or FAV in multiple cell types.
(A-C) Dose-dependent antiviral activity of RBV, FAV and NHC. Cells were treated with different concentrations of either RBV or FAV or NHC or DMSO (vehicle control/VC) immediately after virus adsorption to the cells. At 24 hr post-infection (hpi), virus titer was measured in the cell supernatant by plaque assay. Each line graph represents the mean ± s.d. of infectious viruses present in two individual wells in each of two independent experiments (four wells total) per cell type. (A) Titration of virus produced from drug-treated Vero cells; (B) Titration of virus produced from drug-treated N2a cells; (C) Titration of virus produced from drug-treated hNSC cells; (D) The EC50 (median effective concentration) was determined by extrapolating the dose-response curve of either RBV or FAV or NHC in Vero, N2a cells, and hNSCs.