Skip to main content
. 2024 Sep 30;20(9):e1012574. doi: 10.1371/journal.ppat.1012574

Fig 5. Serial passage of LACV in the presence of increasing NHC concentrations to select for resistance.

Fig 5

(A) Study protocol of serial passaging of LACV in presence of DMSO or increasing concentrations of NHC (0.1 to 0.8 μM), with virus being passaged five times at each concentration. (B) Dose-dependent antiviral activity of NHC tested against the serially passaged LACV. Passaged viruses were used to infect Vero cells treated with different concentrations of NHC or with DMSO (VC). At 24 hr post infection (hpi), virus titer was measured from cell supernatant using a plaque assay. Each line graph represents the mean number of infectious viruses present in three individual wells of an independent experiment with an error bar indicating ± s.d. (C) After 20 passages the virus pools from passage with (n = 207) or without (n = 122) NHC were used to form plaques on Vero cells. Plaque sizes were imaged and their sizes measured; the distributions of plaque sizes for each virus group with the size distributions significantly different assessed as in Fig 2.