FIGURE 3.

A Major structural change in IMT results in IMT isomer 1 (IMTi-1), which retains IMT’s Aβ-reducing effect and its reduction of BACE processing of APP in N2a 695 cells. (A) Structures of IMT and isomer-1, IMTi-1. (B) Western blots of N2a cell lysates (upper) and cell media (lower) from experiments using IMT and IMTi-1 probed with antibody RU369 (anti-C terminal APP) and RU anti-C-terminal sAPPβ (bottom), respectively. Each western blot panel shows lanes from a single gel. However, the three lanes at the right of each, which refer to incubation with IMTi-1, are from a different part of the same gel. (C) Quantification of secreted Aβ40 in N2a cells incubated with IMT or IMTi-1, One-way Anova, p < 0.001. (D) Quantification of sAPPβ levels. One-way Anova, p < 0.001.