Skip to main content
. 2001 Jun;75(11):5315–5327. doi: 10.1128/JVI.75.11.5315-5327.2001

FIG. 3.

FIG. 3

Biological characterization of MHV-76. (A) Single-step growth curves of MHV-68 (▪) and MHV-76 (○) on BHK-21 cells at an MOI of 5. Data are shown as mean log10 virus titer ± standard error and are representative of two separate experiments, each carried out in duplicate. (B) Viral replication in the lungs of BALB/c mice infected intranasally with 2 × 105 PFU of MHV-68 (▪) or MHV-76 (○). The mean log10 virus titer ± standard error for four mice per group is shown for each time point. (C) Numbers of spleen cells during intranasal infection of BALB/c mice with 2 × 105 PFU of MHV-68 (▪) or MHV-76 (○). The mean total number of splenocytes ± standard error for four mice per group is shown for each time point. (D) Latent virus in the spleens of BALB/c mice infected with 2 × 105 PFU of MHV-68 (▪) or MHV-76 (○) as determined by infective-center assay. Infectious virus titers (less than 50 PFU per spleen) were subtracted from the infective-center results. The mean number of infective centers per spleen ± standard error for four mice per group is shown for each time point.