FIG. 4.

Effect of tagged ICP0 and homologues on exogenously expressed ubiquitin precursor. HEp-2 cells were cotransfected with pCIQEUb52 (expressing the tagged ubiquitin precursor Ub52) and either vector alone (A and B) or the UL30-tagged ICP0 homologues as indicated (C through L). At 24 h posttransfection, cells were processed for confocal microscopy and costained with MAb MRGS at a dilution of 1/1,000 (A, C, E, G, I, and K) and polyclonal anti-UL30 r113 at a dilution of 1/3,500 (D, F, H, J, and L). Secondary antibodies used were FITC-conjugated goat anti-rabbit IgG (Sigma) at 1/100 and Cy3-conjugated goat anti-mouse IgG (Amersham) at 1/1,000.