Fig. 2.

HAP-1 enhances the rate of production of both capsid and intermediate. (A) Kinetics of assembly were observed by 90° LS at 400 nm. Final concentrations of HAP-1 ranged from 0 to 20 μM, as indicated. (B) Assembly reactions performed in the presence of 0, 2.0, or 5.0 μM HAP-1 were subjected to SEC at the times indicated to determine the proportions of capsid, intermediate elution, and residual dimer (defined in Materials and Methods). The increased LS at higher [HAP-1] in A arises from increased capsid production and accumulation of intermediates. Assembly reactions contained 11 μM Cp149 dimer and were performed at 37°C in 150 mM NaCl at pH 7.5.