Fig. 3.

HAP-1 can increase the extent of assembly and alters assembly products. Assembly reactions performed as described in the legend of Fig. 2 were subjected to SEC after reaching equilibrium (≈24 h). (A) Representative chromatograms are shown. (B) Data from assembly reactions performed in triplicate are summarized. At higher HAP-1 concentrations, the amount of dimer progressively decreased, and the average size and amount of the assembly products increased (A Inset). At low [HAP], no change in free dimer concentration was observed. (C) Electron micrographs show an increase in the proportion of misshapen particles with increasing [HAP-1]. Assembly reactions for EM were performed at 21°C and contained 5 μM Cp149 and 0.5 M NaCl; each micrograph is labeled with the respective [HAP-1] (in micromolar).