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. 2005 May 25;102(23):8168–8173. doi: 10.1073/pnas.0500735102

Fig. 2.

Fig. 2.

Identification of a [2Fe-2S] cluster in Grx2 by Mössbauer spectroscopy. Mössbauer spectra of human Grx2 (3.4 mM) recorded at 80 and at 4.2 K with B = 4 T applied perpendicular to the γ-beam, respectively. The solid line in the zero field spectrum is a fit with a symmetric Lorentzian doublet with isomer shift δ = 0.27 mm·s-1 and quadrupole splitting ΔEQ = 0.60 mm·s-1, whereas in the applied field spectrum, a simulation is shown for S = 0 with parameters δ = 0.27 mm·s-1, ΔEQ = +0.61 mm·s-1, asymmetry parameter η = 0.6. These parameters indicate the presence of iron in tetrahedral sulfur coordination with pure valence state (III) and total spin state S = 0 characteristic of a [2Fe-2S]2+ cluster.