Fig. 4.

Kinetics of the chromophore loss of Grx2 upon treatment with various compounds. Holo Grx2 (45 μM [Fe-S] center) in 50 mM sodium phosphate, pH 8/300 mM NaCl was incubated with 2 mM of the indicated compounds at 25°C. The loss of the chromophore was recorded after the decrease in absorbance at 428 nm.