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. 2005 May 27;102(23):8333–8338. doi: 10.1073/pnas.0500989102

Fig. 3.

Fig. 3.

Immunofluorescence shows the expression of cyclin D1 and active caspase 3 in cortical neurons after TBI, and effects of flavopiridol treatment. (a) Double immunofluorescence in coronal sections of the brain cortex around the injury site shows staining for cyclin D1 in vehicle, and flavopiridol-treated animal after TBI, and for the neuronal marker NeuN. Cyclin D1 expression is induced in cortical neurons after TBI and strongly reduced after flavopiridol compared with vehicle (arrows). (Original magnification: ×125.) Bar graphs show quantitation of cortical neurons expressing cyclin D1 in ipsilateral and contralateral cortex in vehicle-treated rats, compared with flavopiridol (ipsilateral) (**, P < 0.01). (b) Double immunofluorescence in coronal sections of the brain cortex around the injury site shows staining for cyclin D1 in vehicle, and flavopiridol-treated animal after TBI, and for active caspase 3. Flavopiridol strongly reduces cyclin D1-positive and active caspase 3-positive neurons (arrows). (Original magnification: ×125.) Bar graphs show quantitation of cortical neurons coexpressing cyclin D1 and cleaved caspase 3 in ipsilateral and contralateral cortex in vehicle-treated rats, compared with flavopiridol (ipsilateral) (**, P < 0.01).