Fig. 3.

PDGFR signaling through a single downstream effector is not sufficient for mesoderm cell survival. (A and B) Embryos were coinjected with mRNA encoding β-gal and iPDGFRα or β-gal, PDGFR-37 (R37), and the following iPDGFRαs. (C and D) wt. (E and F) Y572/74. (G and H) Y720. (I and J) Y731/42. (K and L) Y762. (M and N) Y988. (O and P) Y1018. At the beginning of gastrulation (stage 10), AP1510 or DMSO was injected into the blastocoel. At the midgastrula stage (stage 11), β-gal expression was visualized (shown in blue). Representative saggital sections of these embryos are shown. (E–P) Note that single effectors do not restore cell survival. Arrowheads indicate apoptotic mesoderm cells outside the blastocoel cavity.