Fig. 4.

PDGFR signaling through PLCγ and PI3K is required for mesoderm cell survival. (A and B) Embryos were coinjected with mRNA encoding β-gal and iPDGFRα or β-gal, PDGFR-37 (R37), and the following iPDGFRαs. (C and D) wt. (E and F) F720/762. (G and H) F4. At the beginning of gastrulation (stage 10), AP1510 or DMSO was injected into the blastocoel. At the midgastrula stage (stage 11), β-gal expression was visualized (shown in blue). Representative saggital sections of these embryos are shown. As shown in E and F, only the presence of PLCγ, PI3K, and Src pYBs are required to restore mesoderm cell survival.