Fig. 5.

PDGFR signaling through PLCγ and PI3K is required for mesoderm cell survival. The data presented in Figs. 2, 3, 4 are shown in graph form. The percentage of embryos that do not contain apoptotic cells (i.e., cells with nonnuclear β-gal staining) is presented for embryos injected with mRNA as described in Figs. 2, 3, 4 and with DMSO (gray bars) or AP1510 (black bars). This percentage is low for some mutants compared with wt because there may be some basal activity of the receptor construct without the addition of dimerizer. Error bars represent standard error and were calculated from a minimum of three separate experiments. The data used to construct this graph is available in Table 1, which is published as supporting information on the PNAS web site.