Figure 4.

P2Y₁₂ Inhibition Suppresses IFN-Associated MK Activation

MKs were pretreated with a P2Y₁₂ inhibitor for 20 hours, stimulated with IFNα for another 4 hours, and lysed. (A-D) Levels of IFNα proteins were measured using real-time polymerase chain reaction (n = 7) (A, B) and immunoblot densitometry (n = 4) (C, D). Data presented as mean ± SD and compared using 2-sided paired Student's t-tests. ∗P < 0.05; ∗∗P < 0.01. DMSO = dimethyl sulfoxide; PBS = phosphate-buffered saline; other abbreviations as in Figure 1.