Figure 5.

P2Y₁₂ Inhibition Suppresses MTOR Signaling Pathway

(A, B) Bar graphs of differentially expressed MTOR-related genes identified in MKs (A) and platelets (B) treated with P2Y₁₂ inhibitor (P < 0.05). (C) Venn diagram of overlapping MTOR-related genes between MKs and platelets. Heatmap of differentially expressed MTOR pathway genes identified in MKs and platelets treated with P2Y₁₂ inhibitor (P < 0.05). (D to G) MEG-01 cells were treated with 2.5 μmol/L torin-1 for 24 hours and lysed. Protein levels (D) of (E) phospho-RPS6 (F) IFITM3 and (G) ISG15 were measured using immunoblot densitometry (n = 6). Data presented as mean ± SD and compared using 2-sided unpaired Student's t-tests. ∗P < 0.05; ∗∗P < 0.01. Abbreviations as in Figures 1 and 4.