Fig. 1.

Detection of 5040 plant proteins in agroinfiltrated Nicotiana benthamiana leaves. (a) Total extracts from agroinfiltrated N. benthamiana leaves were separated by SDS‐PAGE and the lane was cut into 24 slices. Proteins in each gel slice were digested with trypsin and analyzed by LC‐MS/MS. The spectra detected in the replicates were combined for each gel slice for further analysis. (b) Number of peptides per protein. Spectra were mapped to the LAB3.60 annotation of the N. benthamiana proteome and the number of unique and non‐unique peptides were plotted for each protein. (c) Correlation between predicted and apparent molecular weight (MW). For each protein, the apparent MW was plotted against the predicted MW. The predicted MW was calculated after subtracting the putative signal peptide. (d) Number of gel slices in which each protein was detected, not including adjacent gel slices.