TABLE 1.
Summary of plasmids used in this study
| Plasmida | Encoded polypeptide | Amino acid substitutedb/relevant RNA sequencec |
|---|---|---|
| pB1TP3 | BMV wild-type 1a | |
| pB2TP5 | BMV wild-type 2a | |
| pB3TP8 | BMV wild-type 3a and wild type CP | |
| pB3TP8-derived mutants | BMV wild-type 3a and CP variant | |
| pBSK052053AA | SK052053AA | 52(S), 53 (K)/UCA AAG→GCA GCG |
| pB3SS078079AA | SS078079AA | 78(S), 79 (S)/UCU UCU→GCU GCU |
| pB3NK082083AA | NK082083AA | 82(N), 83 (K)/AAU AAG→GCU GCG |
| pB3EQ110112AA | EQ110112AA | 110(E), 112 (Q)/GAG AAA CAG→GCG AAA GCG |
| pB3SS128129AA | SS128129AA | 128(S), 129 (S)/UCC UCG→GCC GCG |
| pB3YL155156AA | YL155156AA | 155(Y), 156 (L)/UAU CUG→GCU GCG |
| pB3HV175176AA | HV175176AA | 175(H), 176 (V)/CAC GUA→GCC GCA |
| pB3P178A | P178A | 178 (P)/CCU→GCU |
| pB3T179A | T179A | 179 (T)/ACG→GCG |
| pB3F180A | F180A | 180 (F)/UUC→GCC |
| pB3D181A | D181A | 181 (D)/GAU→GCU |
| pB3D182A | D182A | 182 (D)/GAC→GCC |
| pB3F183A | F183A | 183 (F)/UUC→GCC |
| pB3F184A | F184A | 184 (F)/UUC→GCC |
| pB3T185A | T185A | 185 (T)/ACC→GCC |
| pB3P186A | P186A | 186 (P)/CCG→GCG |
| pB3V187A | V187A | 187 (V)/GUU→GCU |
| pB3Y188A | Y188A | 188 (Y)/UAU→GCU |
| pB3R189A | R189A | 189 (R)/AGG→GCG |
a
pB1TP3, pB2TP5, and pB3TP8 were described in detail previously (22).
b
The amino acid residues substituted for with alanine are shown by the standard one-letter codes in parentheses accompanied by the numbers that indicate their position in BCP.
c
The short RNA sequences represent codon changes that induce amino acid substitutions. As an exception, the mutant EQ110112AA accompanies the unchanged AAA codon.