TABLE 2.
Analyses of BCP mutants bearing amino aid substitution in the putative internal surface regions
| BCP mutanta | Encapsidation (arbitrary unit)b | Movementc
|
Virus yield (mg/g FW)d | |
|---|---|---|---|---|
| I | S | |||
| Mock | 0.0 | − | − | 0.0 ± 0.0 |
| B3SK052053AA | 0.0 | − | − | 0.0 ± 0.0 |
| B3SS078079AA | 1.1 | + | + | 2.5 ± 0.1 |
| B3NK082083AA | 0.8 | + | + | 1.3 ± 0.2 |
| B3EQ110112AA | 0.3 | + | + | 1.4 ± 0.0 |
| B3SS128129AA | 0.0 | − | − | 0.0 ± 0.0 |
| B3YL155156AA | 0.0 | − | − | 0.0 ± 0.0 |
| B3HV175176AA | 0.0 | − | − | 0.0 ± 0.0 |
| Wild-type B3 | 1.0 | + | + | 4.0 ± 0.3 |
Wild-type BMV RNA3 or its derivatives were used as the inoculum together with wild-type BMV RNAs 1 and 2.
The Northern blotting data in Fig. 2 and from two other experiments were densitometrically analyzed, averaged, and indicated as an aribtrary unit.
The transcripts were inoculated on 6-day-old barley seedlings, and virus infectivity was measured by the existence of viral RNAs 2 weeks after inoculation. BMV RNAs in inoculated (I) and systemic (S) leaves were detected by tissue printing assay (31).
CP accumulation in secondary leaves (systemic leaves) was measured by ELISA and expressed as virion concentration. Data are means ± standard deviation for three replicates. FW, fresh weight.