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UPLC-HRMS analysis of formic acid formed from 14α-CD18O dihydrolanosterol by human P450 51A1 in H218O. Traces are shown for (A) no 18O (only 16O, m/z 167.0925), (B) one 18O atom (m/z 169.0968), and (C) two 18O atoms (m/z 171.1010). Isotopically labelled oxygen atoms (18O) are indicated in blue, and all m/z values are calculated for the derivatized pyridyl formate esters. (The corresponding raw chromatographic traces are presented in Figure S6).
