Fig. 3.

ARV or UV-ARV upregulates the IFN-γ expression levels in P-PBMCs. (A) IFN-γ expression by P-PBMCs after ARV or UV-ARV stimulation. P-PBMCs were sensitized with ARV or UV-ARV. Intracellular IFN-γ levels were analyzed 24 h post treatment later in CD3⁺ and CD56⁺ cells using two-color flow cytometry. (B) P-PBMCs cultured with 5 µg/ml anti-IFN-γ Ab or isotype control Ab for 1 h followed by sensitization with ARV and cultured for 24 h. Representative results for CD3⁺ and CD56⁺ cells are shown in histograms based on at least 10⁴ gated cells. RMFI is shown on histograms. Similar results were observed using 3 different P-PBMC donors. (C) Decreasing numbers of P-PBMCs (ranging10⁷to10⁴) were stimulated with ARV or UV-ARV, respectively. After stimulated, IFN-γ levels in the culture supernatants were determined by ELISA