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. 2024 Oct 21;22:514. doi: 10.1186/s12964-024-01888-0

Fig. 5.

Fig. 5

ARV or UV-ARV induces most immunogenic apoptosis in PGC cells (PGCs) and upregulates the TRAIL expression levels by CD8+TILs. CD8+T cells, CD4+ T cells, CD56+NK cells, and CD14+monocyte/macrophages were co-cultured with PGCs followed by sensitization with ARV or UV-ARV for 24 h and 48 h, respectively. The ratio of coculture cell numbers of TILs and PGCs was 5:1. Cell death was measured by LDH cytotoxicity assay (A) and DELFIA EuTDA cytotoxicity detection (B). Data represent the mean of triplicate experiments, and experiments were repeated at three times using different donor TILs with similar results. (C) The expression levels of TRAIL were analyzed on CD8+ TILs 24 h post treatment ARV or UV-ARV using two-color flow cytometry. Cell viability was > 95%, as assessed by PI exclusion. Similar results were observed using at least 3 different CD8+ TILs donors. (D) CD8+TILs cultured with 5 µg/ml anti-IFN-γ Ab or isotype control Ab for 1 h, followed by sensitization with ARV or UV-ARV and cultured for 24 h. Representative results for CD8+ TILs are shown in histograms, RMFI is shown on histograms. Similar results were observed using 3 different CD8+ TILs donors