Fig. 1.

Sequence variation affects enzyme properties. (A) SDS/PAGE comparing the expression and solubility of randomly selected, shuffled GAT variants with those of the three WT enzymes. (B) Pairwise amino acid identity expressed as the number of differences for 11 enzymes that were selected for crystallization trials. Shading is from fewest (light gray) to most (dark gray) differences. (C) Side-by-side comparison of the behavior of the three WT and eight variant enzymes in the HT crystal screen condition yielding the best unoptimized crystals for variant 5.