TABLE 2.
Infection rates of Cx. quinquefasciatus midguts, legs and wings, salivary glands, and saliva infected with WNV and SLEVa
| Sample type | Infection | Day 7 | Day 14 | ||
|---|---|---|---|---|---|
| WNV | SLEV | WNV | SLEV | ||
| Midgut | WNV | 100% (32/32) | – | 100% (32/32) | – |
| SLEV | – | 97% (30/31) | – | 97% (29/30) | |
| WNV + SLEV | 94% (29/31) | 90% (28/31) | 100% (32/32) | 97% (31/32) | |
| Legs and wings | WNV | 71% (22/31) | – | 97% (31/32) | – |
| SLEV | – | 16% (5/32) | – | 78% (25/32) | |
| WNV + SLEV | 59% (19/32) | 19% (6/32) | 97% (31/32) | 66% (21/32) | |
| Salivary glands | WNV | 72% (23/32) | – | 81% (26/32) | – |
| SLEV | – | 16% (5/32) | – | 38% (12/32) | |
| WNV + SLEV | 41% (13/32)* | 16% (5/32) | 81% (26/32) | 41% (13/32) | |
| Saliva | WNV | 44% (14/32) | – | 44% (14/32) | – |
| SLEV | – | 3% (1/32) | – | 13% (4/32) | |
| WNV + SLEV | 22% (7/32) | 3% (1/32) | 60% (19/32) | 19% (6/32) | |
a
Infection rates were determined by detection of viral RNA via qRT-PCR. Significant differences between single and coinfection for each virus, sample, and time point using a chi-square test are shown (*P < 0.05). – indicates samples that were not tested.