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. 2024 Oct 22;12:RP88318. doi: 10.7554/eLife.88318

Figure 1. Dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) regulates cell size.

shRNA-mediated knockdown of DYRK1A was performed in (A, B) HEK293 and (C, D) SH-SY5Y cells using lentivirus. Transduced cells were selected for four days before analysis. Western blot shows the efficiency of DYRK1A knockdown. (E, F) NIH3T3 cells were treated with Dyrk1a-targeting sgRNA expressing lentivirus and selected for four days before analysis. Western blot shows the efficiency of DYRK1A knockdown. (G, H) HEK293 cells expressing Flag-DYRK1A and the parental cells were treated with 40ng/ml Doxycycline for 48hr and analyzed for cell size. (G) Overexpression was analyzed by qRT-PCR. GAPDH mRNA was used to normalize RNA in q-RT-PCR samples. Data represent the mean ± SD (n=3 biological replicates).

Figure 1—source data 1. Uncropped blots for Figure 1A.
Figure 1—source data 2. Raw blots showing knockdown of DYRK1A for Figure 1A.
Figure 1—source data 3. Uncropped blots for Figure 1C.
Figure 1—source data 4. Raw blots showing knockdown of DYRK1A for Figure 1C.
Figure 1—source data 5. Uncropped blots for Figure 1E.
Figure 1—source data 6. Raw blots showing knockdown of Dyrk1a for Figure 1E.

Figure 1.

Figure 1—figure supplement 1. Analysis of cell size after induction of dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A) expression with increasing dosage of Doxycycline.

Figure 1—figure supplement 1.