Fig. 4. Expression levels of LHβ (A), FSHβ (B) and pgrl (C) in the pituitary cells from control, NaCl, SPE, and NaCl+SPE groups.

Relative abundance of the mRNA was measured by the comparative threshold cycle method using qRT-PCR and normalized the amount of β-actin mRNA. Results are expressed as means±SEM (n=3–4). Different letters above the bars represent significant differences (p<0.05). SPE, salmon pituitary extract; qRT-PCR, quantitative real-time PCR; LHβ, luteinizing hormone; FSHβ, follicle stimulating hormone; pgrl, progesterone receptor-like.