Table 2. Primers used for quantitative real-time PCR (qRT-PCR) for reproduction and/or salinity related genes of eel pituitary cell.
| Gene | Primer sequence | Product size | |
|---|---|---|---|
| β-actin | F | GAGACCTTCAACACCCCAG | 95 |
| R | CCAGAGTCCATCACAATACCAG | ||
| LHβ | F | GATCCCCATGTGACTTTCCC | 72 |
| R | GCAGTCAGACGTGTCCATG | ||
| FSHβ | F | CTCGCCAACATCTCCATCTC | 99 |
| R | AGAATCCTGGGTGAAGCAC | ||
| pgrl | F | GGGTAGTGACAGTGAGATTTGG | 116 |
| R | CAGCCCAAGACAAATTTTCGG | ||
| PRL | F | GATTCACAAGATTGGCTCCTC | 143 |
| R | TTGTCGATTTTGTGGGAGTC | ||
| drd4 | F | CGGATTTGGGACTGTACTTCTG | 92 |
| R | TCGCTTGAGTGATATACGCAC | ||
| NPBWR2 | F | AGTTGTATGCCTTCGGTTCC | 126 |
| R | TCAATCCCACGGCACATATG | ||
| rxfp3-2b | F | GTAATCTGTCCGCAACCAAAAG | 102 |
| R | GACTGACAATAAAGTTCACGGTG |
LHβ, luteinizing hormone; FSHβ, follicle stimulating hormone; pgrl, progesterone receptor-like; PRL, prolactin; drd4, dopamine receptor D4; NPBWR2, neuropeptide B/W receptor 2; rxfp3-2b, relaxin family peptide receptor 3-2b.